mouse calvarial osteoblastic mc3t3-e1 cells Search Results


cell lines mc3t3 e1  (ATCC)
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ATCC cell lines mc3t3 e1
Cell Lines Mc3t3 E1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse osteoblastic cells  (ATCC)
97
ATCC mouse osteoblastic cells
Mouse Osteoblastic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse osteoblastic cells - by Bioz Stars, 2026-09
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experimental design pre osteoblast mouse calvarial cells  (ATCC)
99
ATCC experimental design pre osteoblast mouse calvarial cells
Experimental Design Pre Osteoblast Mouse Calvarial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/L+Cells/pmc05899926-118-5-12
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experimental design pre osteoblast mouse calvarial cells - by Bioz Stars, 2026-09
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mc3t3 e1 cell line  (ATCC)
96
ATCC mc3t3 e1 cell line
Mc3t3 E1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/MC3T3-E1+Subclone+24/pmc09638960-81-7-13
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mc3t3 e1 cell line - by Bioz Stars, 2026-09
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mouse preosteoblastic mc3t3-e1 cells gdc0188  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection mouse preosteoblastic mc3t3-e1 cells gdc0188
Mouse Preosteoblastic Mc3t3 E1 Cells Gdc0188, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/mc3t3+e1+cells/10__1155_slash_2023_slash_8857436-48-0-10
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mouse osteoblastic mc3t3-e1 cells  (BioResource International Inc)
90
BioResource International Inc mouse osteoblastic mc3t3-e1 cells
Mouse Osteoblastic Mc3t3 E1 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/mc3t3+e1+cells/pmc06209325-68-0-5
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mc3t3 e1 cells  (ATCC)
96
ATCC mc3t3 e1 cells
Mc3t3 E1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/MC3T3-E1+Subclone+14/pmc04276739-137-0-14
Average 96 stars, based on 1 article reviews
mc3t3 e1 cells - by Bioz Stars, 2026-09
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mc3t3 e1 cells  (OriGene)
91
OriGene mc3t3 e1 cells
Osteoblast differentiation in Tmem161a-overexpressing <t>MC3T3-e1</t> cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).
Mc3t3 E1 Cells, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smad1 phosphorylation western material mc3t3 e1 mouse osteoblast  (Novartis)
86
Novartis smad1 phosphorylation western material mc3t3 e1 mouse osteoblast
Osteoblast differentiation in Tmem161a-overexpressing <t>MC3T3-e1</t> cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).
Smad1 Phosphorylation Western Material Mc3t3 E1 Mouse Osteoblast, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse osteoblast like mc3t3 e1 cells  (Procell Inc)
86
Procell Inc mouse osteoblast like mc3t3 e1 cells
Osteoblast differentiation in Tmem161a-overexpressing <t>MC3T3-e1</t> cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).
Mouse Osteoblast Like Mc3t3 E1 Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/cells+e1+mc3t3/10__1016_slash_j__ceramint__2024__06__156-64-0-4
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mus musculus mouse calvaria preosteoblast cells line calvaria preosteoblast cells line mc3t3e1 cells  (ATCC)
99
ATCC mus musculus mouse calvaria preosteoblast cells line calvaria preosteoblast cells line mc3t3e1 cells
Osteoblast differentiation in Tmem161a-overexpressing <t>MC3T3-e1</t> cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).
Mus Musculus Mouse Calvaria Preosteoblast Cells Line Calvaria Preosteoblast Cells Line Mc3t3e1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+calvarial+osteoblastic+mc3t3-e1+cells/RAW+264%2E7/10__1016_slash_j__jot__2020__02__001-122-1-10
Average 99 stars, based on 1 article reviews
mus musculus mouse calvaria preosteoblast cells line calvaria preosteoblast cells line mc3t3e1 cells - by Bioz Stars, 2026-09
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Osteoblast differentiation in Tmem161a-overexpressing MC3T3-e1 cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).

Journal: Scientific Reports

Article Title: Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway

doi: 10.1038/s41598-023-41837-4

Figure Lengend Snippet: Osteoblast differentiation in Tmem161a-overexpressing MC3T3-e1 cells. ( a ) Expression of Tmem161a in MC3T3-e1 cells during osteoblast differentiation. ( b ) Fluorescence detection of GFP-tagged Tmem161a 1 day after transfection. Cell nuclei were stained with DAPI. Scale bar 100 μm (right), 50 μm (left). ( c ) Immunocytochemistry analysis of Tmem161a after transfection with an overexpression vector. ( d ) qPCR analysis of Tmem161a mRNA in MC3T3-e1 in control and Tmem161a-overexpression vector-transfected cells. qPCR analysis of Alp mRNA ( e ) and Osx mRNA ( f ). ( g ) ALP staining in MC3T3-e1 cells 5 days after transfection with control or Tmem161a-overexpression vectors. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).

Article Snippet: MC3T3-e1 cells were electroporated using a Tmem161a-GFP-tagged plasmid vector (#MG207689, OriGene, Rockville, MD, USA) and Tmem161a-Flag-tagged plasmid vector (#MR207689, OriGene) or empty vector using NEPA21 (Nepa Gene, Chiba, Japan).

Techniques: Expressing, Fluorescence, Transfection, Staining, Immunocytochemistry, Over Expression, Plasmid Preparation, Control

Osteoblast differentiation in Tmem161a-knockdown and -KO MC3T3-e1 cells. ( a ) qPCR analysis of Tmem161a mRNA in control and Tmem161a siRNA-transfected cells. qPCR analysis of Alp mRNA ( b ) and Osx mRNA ( c ) . ( d ) ALP staining in MC3T3-e1 cells 5 days after transfection. Osteoblast differentiation in Tmem161a-KO MC3T3-e1 cells generated using CRISPR/Cas9. ( e ) Sequencing of WT and KO cells. KO cells exhibited 4–base pair and 17–base pair deletions. qPCR analysis of Alp mRNA ( f ) and Osx mRNA ( g ) in WT and Tmem161a-KO cells during osteogenesis. ( h ) Staining of ALP and Alizarin Red S. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05).

Journal: Scientific Reports

Article Title: Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway

doi: 10.1038/s41598-023-41837-4

Figure Lengend Snippet: Osteoblast differentiation in Tmem161a-knockdown and -KO MC3T3-e1 cells. ( a ) qPCR analysis of Tmem161a mRNA in control and Tmem161a siRNA-transfected cells. qPCR analysis of Alp mRNA ( b ) and Osx mRNA ( c ) . ( d ) ALP staining in MC3T3-e1 cells 5 days after transfection. Osteoblast differentiation in Tmem161a-KO MC3T3-e1 cells generated using CRISPR/Cas9. ( e ) Sequencing of WT and KO cells. KO cells exhibited 4–base pair and 17–base pair deletions. qPCR analysis of Alp mRNA ( f ) and Osx mRNA ( g ) in WT and Tmem161a-KO cells during osteogenesis. ( h ) Staining of ALP and Alizarin Red S. Data represent the mean ± SD from 3 independent experiments. Asterisks indicate statistically significant differences (*p < 0.05).

Article Snippet: MC3T3-e1 cells were electroporated using a Tmem161a-GFP-tagged plasmid vector (#MG207689, OriGene, Rockville, MD, USA) and Tmem161a-Flag-tagged plasmid vector (#MR207689, OriGene) or empty vector using NEPA21 (Nepa Gene, Chiba, Japan).

Techniques: Knockdown, Control, Transfection, Staining, Generated, CRISPR, Sequencing